Rebecca Night was born in Poole, Dorset. She attended Yarrells Preparatory School in Upton, Dorset where she took part in the annual musical productions, and later Parkstone Grammar School in Poole as well as Brownsea Open Air Theatre. Night is a former member of the National Youth Theatre, where she appeared as Hero in Much Ado About Nothing and in Master & Margarita at the Lyric Hammersmith. She later trained at Rose Bruford College.
She is married to fellow actor Harry Hadden-Paton, whom she met while performing The Importance of Being Earnest.