Johnny Test features special guest stars in the series have included Lance Bass, Fred Willard, Penn Jilette, Jane Lynch, Jon Polito, Brian George, Snoop Dogg, James Belushi, Kathy Najimy, Ice-T, Dale Earnhardt Jr. and Bob Glouberman. Every episode contains 2 11-minute story segments and 2 segments including "May I Have a Word?" and "Silly Songs with Johnny".
